The Afek laboratory’s overarching goal is to obtain a deep molecular understanding of protein-DNA interactions involved in processes that are fundamental to physiology, disease, and evolution. We are developing cutting-edge approaches to manipulate DNA molecules in high throughput by introducing chemical and mechanical DNA perturbations that frequently occur in the human genome, and we use it to bridge the gap between test tubes and living cells. We use multidisciplinary approaches that closely integrate novel experimental and computational methods.